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A direct monoclonal antibody sandwich immunoassay for detection of Bonamia ostreae (Ascetospora) in hemolymph samples of the flat oyster Ostrea edulis (Mollusca : Bivalvia) ArchiMer
Cochennec, Nathalie; Hervio, Dominique; Panatier, B; Boulo, Viviane; Mialhe, Eric; Rogier, H; Grizel, Henri; Paolucci, F.
Currently recognition of molluscan diseases relies heavily upon light microscopic examination of stained histological sections of molluscan tissues Such histopathological methods are time consuming and relatively inefficient In the study of ongoing epizootics Alternatively, more effluent investigative tools are needed to accelerate disease recognition and quantification Such methods are needed to prevent control and eradicate molluscan diseases This paper reports the development of a direct sandwich enzyme-lmmunosorbent assay (ELISA) for the ascetosporan intrahemocytic parasite Bonamiao ostreae of the flat oyster Ostrea edulis. Bonamiasis is a commercially important epizootic disease of oysters A Bonamia-specific monoclonal antibody is employed in the...
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Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/publication-2765.pdf
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An infection of Ruditapes decussatus (Bivalvia) by Rickettsia ArchiMer
Mialhe, Eric; Chagot, Dominique; Boulo, Viviane; Comps, Michel; Ruano, Francisco; Grizel, Henri.
The authors report the presence of a Rickettsia in Ruditapes decussatus from natural populations of the Algarve (Portugal). Light microscopic shows infections of varying importance in gill tissues. Some enclose small procaryotic colonies (cp) scattered in all lamellae; in others, the colonies are scarce and hypertrophic (100 mu m). These different pictures suggest an amplification process. Digestive diverticulae may also be infected, but to a lesser extent. The ultrastructural features of this intravacuolar (mvd: densified vacuolar membrane) procaryote (P) and its multiplication by scission without a complex developmental cycle allows its classification in the order Rickettsiales.
Tipo: Text Palavras-chave: Ruditapes philippinarum; Pathogens; Histopathology; Pathology.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/1987/acte-3107.pdf
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Bilan 1999 du Réseau REPAMO - Réseau National de Surveillance zoosanitaire des Mollusques marins ArchiMer
Thebault, Anne; Martin, Anne-genevieve; Le Coguic, Yvette; Hirata, Toshio; Tige, Gilbert; Pichot, Yves; Grizel, Henri; Berthe, Franck; Cochennec, Nathalie; Renault, Tristan; Le Roux, Frederique.
Le réseau REPAMO (Réseau de Pathologie des Mollusques) est un réseau de santé animale, qui s'occupe des coquillages marins du littoral français métropolitain. Les mollusques marins des côtes françaises représentent une ressource par la pêche, mais sont surtout une part importante de l'aquaculture. Les pertes liées aux maladies dans ce domaine d'activité sont aujourd'hui considérées comme l'un des principaux facteurs limitants de son développement au niveau mondial. Deux exemples récents suffisent à illustrer ce danger pour l'ostréiculture française : • La culture de l''huître plate Ostrea edulis, déjà atteinte par un parasite, Marteilia refringens, est passée en quelques années de 20 000 tonnes à 1800 tonnes, suite à une épizootie. En effet à la fin des...
Tipo: Text Palavras-chave: Réseau surveillance; Pathologie; Mollusques bivalves; Mortalité.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00098/20903/18522.pdf
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Bilan des essais d'acclimatation d'Ostrea chilensis sur les cotes de Bretagne. ArchiMer
Grizel, Henri; Comps, Michel; Raguenes, D; Leborgne, Y; Tige, Gilbert; Martin, A.
Following the mass mortality of Ostrea edulis, some experiments were conducted with O. chilensis broodstock and spat coming from Chiloe Island (Chile). Concurrently, spat of th same species produced in a hatchery was cultivated. After a small period of growth, these oysters died during the summer. Microscopic observations showed Marteilia refringens and Bonamia ostreae in the oysters' tissue. For these reasons, Ostrea chilensis has not been retained to temporarily replace O. edulis.
Tipo: Text Palavras-chave: ANE; France Bonamia ostreae Marteilia refringens Ostrea chilensis Ostrea edulis Bivalvia marine mollusks spat mortality parasites parasitic diseases acclimatization oyster culture.
Ano: 1982 URL: http://archimer.ifremer.fr/doc/1982/publication-1851.pdf
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Bilan du réseau de pathologie des mollusques. REPAMO. 1995 ArchiMer
Grizel, Henri.
Tipo: Text Palavras-chave: Network; Pathology; Molluscs.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/1995/rapport-1924.pdf
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Bilans depuis 1999 et évolution de la stratégie du REPAMO ArchiMer
Thebault, Anne; Martin, Anne-genevieve; Pichot, Yves; Chollet, Bruno; Robert, Maeva; Le Coguic, Yvette; Tige, Gilbert; Hirata, Toshio; Mazurie, Joseph; Le Saux, Jean-claude; Masson, Nadine; Auby, Isabelle; Maurer, Daniele; Le Roux, Frédérique; Cochennec, Nathalie; Renault, Tristan; Berthe, Franck; Grizel, Henri.
The main objectives of the Mollusc Pathology Network (REPAMO) are to prevent introductions or outbreaks of infectious agents, prevent spread within a farm and especially from one farm to another, to study ways of decreasing the impact of infectious agent and to monitor the development of these pathogens in order to guarantee the health status of oysters exchanged with our commercial partners. On the whole, REPAMO's activities have not changed and include: - monitoring the development of diseases that have to be reported to authorities and carrying out analyses to classify zones: bonamiosis and marteiliosis; - baseline monitoring of farmed and wild populations of bivalve molluscs; - studying cases of abnormal mortality events; - controlling mollusc...
Tipo: Text Palavras-chave: Mollusques; Réseau surveillance; Pathologie; REPAMO.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/acte-3265.pdf
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Biotechnologies marines : mythe ou réalité ArchiMer
Grizel, Henri.
En France, la notion de biotechnologies marines (BM) a vu le jour depuis une dizaine d'années sous la poussée d'un petit groupe de chercheurs. Actuellement, et malgré tous les efforts déployés, elle a encore bien du mal à percer dans le monde scientifique et industriel.
Tipo: Text Palavras-chave: France; Recherche; Biotechnologie marine.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/1993/publication-7451.pdf
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Bivale mollusc cell culture ArchiMer
Mialhe, Eric; Boulo, Viviane; Grizel, Henri.
The historical background of bivalve cell culture and its potential uses in molluscan pathology are reviewed. Then, primary cultures for the study of some host-pathogen interactions at the cellular level are considered. Next, alternative methods to classical cell cultures are examined that may lead to the production of bivalve celllines.
Tipo: Text Palavras-chave: Cell culture; Pathology; Molluscs.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/1988/publication-3122.pdf
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Bonamia ostreae, parasite of the European flat oyster, Ostrea edulis, does not experimentally infect the Japanese oyster, Crassostrea gigas ArchiMer
Renault, Tristan; Cochennec, Nathalie; Grizel, Henri.
The protozoan Bonamia ostreae (Ascetospora) is the etiological agent of bonamiosis, or hemocyte disease of the European fiat oyster, Ostrea edulis (Pichot et al., 1980). Since this infection was first reported from the coast of Brittany (Comps et al., 1980), the parasite has spread rapidly along the French Atlantic coasts (Grizel & Tigé, 1982), and in the European countries including the UK, Holland, lreland and Spain (Bannister & Key, 1982; Van Banning, 1982; Polanco et al., 1984). Under the EEC Council Directive 91/67, this parasitic protozoan is included in list II of Annex A. Serious pathogen agents for molluscs that should be obligatorily declared are placed in this list
Tipo: Text Palavras-chave: Infection; Protozoan; Oyster; Ostrea edulis; Bonamia ostreae.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/1995/publication-2892.pdf
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Bonamiasis : a model study of diseases in marine molluscs ArchiMer
Grizel, Henri; Mialhe, Eric; Chagot, Dominique; Boulo, Viviane; Bachere, Evelyne.
Research on bonamiasis, an epizootic disease of the edible oyster Ostrea edu/is caused by the protozoan Bonamia ostreae, is discussed in relation to oyster fanning, research technology, epizootiology, and management. Morphological and infectious characteristics of the parasite are described. Recent progress in isolation and purification of the parasite have permitted investigations into host defense mechanisms, parasite infectivity, and the development of a mol1usc-pathogen mode!.
Tipo: Text Palavras-chave: Epizootic disease; Pathology; Protozoan; Bonamia ostreae; Oysters.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/1988/publication-3120.pdf
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Branchial Rickettsiales-like infection associated with a mass mortality of sea scallop Pecten maximus ArchiMer
Legall, Ghyslaine; Chagot, Dominique; Mialhe, Eric; Grizel, Henri.
A branchial infection of Pecten maximus is histologically and ultrastructurally described. Gill epithelial cells were heavily infected with colonies of procaryotes, believed ta belong to the Rickettsiales.
Tipo: Text
Ano: 1988 URL: http://archimer.ifremer.fr/doc/1988/publication-3125.pdf
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Cell separation by centrifugal elutriation ArchiMer
Bachere, Evelyne; Chagot, Dominique; Grizel, Henri.
There is an ongoing need to isolate and purify ceIls in bivalve mollusc disease research. Many separation techniques differentiate cells solely by a single factor and usually require additives in the medium that may damage live cells. Centrifugai elutriation is a technique that separates particles by size and density simultaneously and can be conducted in physiological media without additives. Living cells can be separated without chemical damage and, because the procedure can be conducted aseptically, cells can be maintained in vitro after elutriation. A description of the technique, methods to determine flow rates and rotor speeds, and a preliminary separation of oyster hyalinocytes is presented
Tipo: Text Palavras-chave: Bivalve mollusc; Centrifugal elutriation; Cell separation.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/1988/publication-3118.pdf
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Compléments d'observations sur le cycle du parasite, Marteilia refringens (Grizel et coll. 1974) ArchiMer
Tige, Gilbert; Grizel, Henri; Langlade, Aime; Rabouin, Marie-agnès.
Les premiers stades connus du parasite ont été mis en évidence en Bretagne pendant les mois de juillet et d'août (GRIZEL et TIGE, 1977). Suite à ces observations, un essai a été réalisé en 1978 pour savoir si l'infestation avait bien lieu à ces dates ou si elle se faisait antérieurement sous une forme non décelée actuellement et subissant des transformations profondes dans les tissus de l'huître. Les résultats montrent que l'infestations se fait effectivement pendant l'été, à partir du mois de juillet jusqu'à la fin du mois d'août. Elle fut dans l'ensemble faible; les lots les plus infestés sont ceux qui ont séjourné le plus longtemps en zone contaminée.
Tipo: Text Palavras-chave: Ostrea edulis; Marteilia refringens; Pathologie; Parasite.
Ano: 1979 URL: http://archimer.ifremer.fr/doc/1979/acte-6098.PDF
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Développement de protistes parasites en culture cellulaire de l'huître japonaise Crassostrea gigas ArchiMer
Cousserans, François; Bonami, Jean-robert; Comps, Michel; Grizel, Henri.
Cell cultures, which are the basis of numerous recent studies in medical pathology, have been, for a few years, of great interest in the studies carried out on the diseases affecting invertebrates. However, such cultures, dealing with marine bivalve molluscs, obtained for the first time by Vago and Chastang (1) and progressively improved since then [(2) to (10)], have not been used in pathological studies yet. As the diseases affecting marine molluscs of economic importance such as oysters are both of great interest and facing specific study difficulties, we tried to adapt oyster cell cultures to the study of the presence, development and cycles of the pathogenic or possibly pathogenic microorganisms affecting Crassostrea, and especially the species gigas,...
Tipo: Text Palavras-chave: Culture cellule; Parasite; Huîtres; Crassostrea gigas.
Ano: 1974 URL: http://archimer.ifremer.fr/doc/1974/publication-5907.PDF
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Développement et cycle du parasite responsable de l'épizootie de l'huître plate Ostrea edulis L. ArchiMer
Grizel, Henri.
The description, in light microscopy, of the different stages of the evolution of the Ostrea edulis digestive gland parasite enabled the highlighting of an annual evolution cycle in sick oyster populations.
Tipo: Text Palavras-chave: Ostrea edulis; Huître plate; Pathologie; Parasitologie.
Ano: 1975 URL: http://archimer.ifremer.fr/doc/1975/acte-6167.PDF
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Données sur la situation de la maladie de l'huître plate, Ostrea edulis ArchiMer
Grizel, Henri; Tige, Gilbert; Rabouin, Marie-agnès.
Au cours des années 1974-1975, la maladie de l'huître plate, Ostrea edulis Linné, a peu à peu gagné la majorité des centres ostréicoles français et provoqué des pertes très importantes tant sur les huîtres d'élevage que sur les gisements naturels. Cette note analyse l'évolution actuelle de l'épizootie ; elle distingue pour cela trois types de centres d'élevage de l'huître plate : les centres anciennement touchés par l'épizootie où la culture traditionnelle de l'huître plate est devenue aléatoire. Elle est remplacée par celle de Crassostrea gigas et par l'affinage, pendant 7 à 8 mois, d'huîtres plates de 3 ou 4 ans qui restent de bonne qualité. Les centres récemment atteints par l'épizootie où, après dragage des bancs naturels parasités, l'élevage d'huîtres...
Tipo: Text Palavras-chave: Ostréiculture; Ostrea edulis; Pathologie; Maladie; Huître plate.
Ano: 1976 URL: http://archimer.ifremer.fr/doc/00009/11999/8692.pdf
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Données sur la situation de la maladie de l'huître plate, Ostrea edulis L. ArchiMer
Grizel, Henri; Tige, Gilbert; Rabouin, Marie-agnès.
Throughout the years 1974-1975, the flat oyster (Ostrea edulis Linné) disease has gradually spread to most of the French oyster culture centres and caused very important losses in farmed oysters and natural deposits. This note analyses the current evolution of the epizooty; to do so, we differentiate three types of flat oyster culture centres: Centres affected in the past by the epizooty and where the traditional flat oyster culture has become uncertain. It has been replaced by the culture of Crassostrea gigas and by the finishing, for 7 to 8 months, of flat oysters aged 3 or 4 years old which remained of good quality. Centres recently touched by the epizooty where the culture of flat oysters (18 months and 2 years old) from unharmed spat is still possible...
Tipo: Text Palavras-chave: Marteilia refringens; Pathologie; Ostrea edulis; Huître.
Ano: 1976 URL: http://archimer.ifremer.fr/doc/1976/acte-5928.PDF
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Données sur le nouveau parasite de l'huître plate. Situation épidémiologique ArchiMer
Tige, Gilbert; Grizel, Henri; Comps, Michel.
Abnormal mortalities of flat oysters, Ostrea edulis, have been monitored in June 1979 in some farms of l'Ile Tudy. The examination of these oysters with light and electron microscopy showed different forms of an intracellular parasite localized in the connective tissue of the gills and the digestive gland. It is also detectable in the blood vessels and the stomachal epithelium. Numerous parasited oysters showed distinctive branchial damages (perforations, indentations) visible through macroscopic examination. The epidemiological monitoring carried out on the Norman and Breton coast revealed the presence of this parasite in a large number of flat oyster farms. The percentage of parasited oysters is higher in areas where mortalities have been monitored but...
Tipo: Text Palavras-chave: Epidémiologie; Huître plate; Ostrea edulis; Mortalité.
Ano: 1980 URL: http://archimer.ifremer.fr/doc/1980/acte-4925.PDF
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Epidémiologie des bivalves ArchiMer
Grizel, Henri.
The notions of pathology and epidemiology in marine aquaculture are relatively new, especially as far as molluscs are concerned, as they've only been used for about thirty years. Although numerous studies have been conducted, and the concepts in these specific fields have evolved, only very few actions are proposed to stop the spreading of a disease. Only a few main mollusc species are cultivated throughout the world and most of them suffered or suffer from epizooties. Today, their pathological history is more solid: several infectious diseases are now known, numerous pathogens or potential pathogens have been described, diverse mortalities have been studied and several types of tumours have been observed. We now live in some cases, as for the flat oyster...
Tipo: Text Palavras-chave: Aquaculture marine; Pathologie; Conchyliculture; Bivalves; Epidémiologie.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-6580.pdf
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Epizootiological study of rickettsiosis of the Saint-Jacques scallop Pecten maximus ArchiMer
Le Gall, G; Mialhe, Eric; Chagot, D; Grizel, Henri.
Rickettsia-like microorganisms were described in gill endothelial cells during mass mortalities of Saint-Jacques scallops Pecten maximus in Saint-Brieuc Bay (North Brittany, France) during winter 1985-86. In order to evaluate the epizootiological significance of rickettsiosis of scallops, an analytical and descriptive epizootiological study was undertaken. The study included the geographic distribution, incidence and severity of the disease for several northern European and French scallop populations. Since the center of French scallop production is in Saint-Brieuc Bay, epizootiological investigations were concentrated in this area. Dissemination appeared to be by horizontal transmission. The pathogenic significance of the rickettsia-like organism in...
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Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/publication-2758.pdf
Registros recuperados: 94
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